Bone Marrow and Stem Cell Transplantation: 134: Beksac, Meral


Olerup Ssp AB, STOCKHOLM Företaget

For each product, product inserts, worksheets and other information can … Cutting edge technology is essential for offering exceptional genotyping services. In 2013, as the world’s first HLA typing lab, we started putting the breakthrough NGS technology, into routine use for HLA typing in a truly high throughput and highly parallelised fashion. Using this process, over 1 million potential stem cell donors are currently typed per year. HLA genes are found on the short arm of chromosome 6 and are highly polymorphic with paternal and maternal alleles being coexpressed in each individual. In 1992, Olerup SSP AB pioneered the field of molecular HLA typing with the introduction of its invention PCR-SSP (PCR amplification with sequence-specific primers) (4).

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Recently, amplification of DNA using sequence‐specific primers (PCR‐SSP) has proved a reliable and rapid method for typing HLA‐DR, HLA‐DQA and HLA‐DQB genes. PCR‐SSP takes two hours to perform and is therefore suitable for the genotyping of cadaveric donors. We describe here a DNAJoaseO Cw typing medxx:l whch resolves the i~oblems of Cw detection by serology.Arnplificetion of DNA using sequence-sbecific primers (PCR-SSP) has proved a reliable and rapid moftmO for typing HLA-DRB1 (1) and DQBt (manuscnlX sul~mitted) genes.PCR-SSP takes two hours to perform and is therefore surtai~e for the genotyping of cadaveric donors. Biotest HLA SSP Kits SSP-Reagenzienkit für die HLA-Typisierung auf DNA-Basis Ready to use SSP reagent kit for DNA based HLA typing Trousse de réactifs SSP pour le typage HLA, basé sur l’ADN Kit di reagenti SSP per tipizzazione HLA basata sul DNA Juego de reactivos SSP para la tipificación del antígeno [IVD] For In Vitro Diagnostic Use Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is Results more widely used to detect HLA-DRb1*04 alleles. Addi- tionally, PCR-SSOP and Luminex methods are described The presence of a 259-bp specific band along with 434-bp as well for identifying the HLA alleles.


2021-03-14 · DNA-based HLA Typing. HLA typing using molecular techniques has now become the clinical standard for patient-donor matching. DNA-based typing does not require live lymphocytes and can be performed using a variety of source materials including whole blood, buccal swabs, biopsy samples and frozen tissue.

Klinisk immunologi Akademiska

We use 9 sequence-specific primers and 2 group specific primers to define the HLA-DRB1 specificities DR1, DR2, DR3, DR4, DR5, DR6, DR7, DR8, DR9 and DR10. The HLA DR3, DR5, DR6 and DR8 can be amplified by the two primers of DR3568 and DRB1. Dalva K., Beksac M. (2007) Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles. In: Beksac M. (eds) Bone Marrow and Stem Cell Transplantation. Methods in Molecular Medicine, vol 134. Humana Press.

Pcr-ssp hla typing

Considering HLA-DR1-10, differences in typing results were found in 3 out of 73 healthy individuals and 8 HLA typing by sequence-specific oligonucleotides probes (PCR-SSOP) Example of hybridization specificity with SSO probes.
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Pcr-ssp hla typing

top. Sequence specific primed PCR (PCR-SSP) HLA typing via sequence specific PCR primers is a flexible and still common method, used mostly with clinical samples where CE-IVD labelling is required. top HLA-Ready Gene - The SSP-PCR System. Integrated detection of the nullalleles A*24:09N, B*51:11N, C*04:09N, DRB4*01:03:01:02N, DRB5*01:08N. Integreated negative control.

DOI; Publisher Name Humana Press Most molecular HLA typing methods are based on the group-specific amplification by PCR where the PCR-SSP technique is widely used to detect HLA-B* 27 .
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Klinisk immunologi Akademiska

HLA-DR typing was performed using standard microcytotoxicity assay and PCR-SSP method in 28 patients referred to our Transplantation Immunology Unit for HLA typing. Comparison of results obtained by both methods revealed no discrepancies in 5 patients, in 12 patients the PCR-SSP typing showed additional DR antigens or splits of antigens. At least two PCR-SSP methods for gen- eric typing of HLA-DR specificities have been pub- lished (4, 5), as has a PCR-SSP method for the Brief communication volume of 20 pl. All reagents but the Taq Poly- merase were pre-mixed and stored at -20°C in order to speed the process of typing. HLA DR typing by PCR-SSP: Advantages and inconveniences after six months of routine use in three laboratories Dominique Charron HLA-A locus specificities identified by Sequence Specific PCRWe have established a system for typing the HLA Class I 'A' locus from genomic DNA, by a one-step polymcrase chain reaction (PCR) based on ARMS (Amplification Refractory Mutation System l).